Ofloxacin and Norfloxacin used in this experiment are widely present in the environment and are not easily degraded. They are easily taken into the body by the organisms in the environment and damage the biological antioxidant system. Norfloxacin and Ofloxacin were formulated into 0、30、60、120 and 360mg/L concentrations to conduct exposure tests on zebrafish and Scenedesmus obliquus. The SOD activity and CAT activity index of zebrafish and Scenedsemus obliquus were measured on the 5th、10th and 15th day of the experiment respectively. To evaluate the damage of Ofloxacin and Norfloxacin to different aquatic organisms.