Optimization of fermentation conditions for Bacillus subtilis against to plant soft rot
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1.Hebei University of Science and Technology,College of Bioscience and Bioengineering,Shijiazhuang,Hebei;2.Agricultural and rural Bureau of Chongli District,Zhangjiakou

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TS202.1

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    Abstract:

    In order to increase bacterial yield and obtain more target protein products, orthogonal method and response surface method were used to optimize the fermentation process of probiotic Bacillus subtilis expression strain Asr. The results showed that the three main factors affecting bacterial yield were leaching yeast powder, MgSO4 and CaCl2. The optimized fermentation medium formulation was: sucrose 20 g/L, peptone 10 g/L, leaching yeast powder 8.65 g/L, K2HPO4 3.0 g/L, MgSO4 0.27 g/L, CaCl2 0.53 g/L. The bacterial yield increased by 1.76-fold compared with that before optimization, with an average of 3.25×108 CFU/mL. After optimum of culture media, the fermentation conditions were optimized by single factor test in 3 L fermentor: the optimal temperature was 37 ℃, the initial pH value was 7.0, the inoculation volume was 10%, and the stirring speed was 250 r/min. The highest yield of Bacillus subtilis Asr reached 7.30×108 CFU/mL, and the test results laid a foundation for the subsequent expansion of fermentation tanks to increase biomass production.

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History
  • Received:June 19,2020
  • Revised:June 19,2020
  • Adopted:September 23,2020
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