To address the lack of understanding of differential volatile compounds in organic and non-organic milk, volatile compounds in organic and non-organic milks from Holstein, Jersey and Yak were comparatively analyzed by gas chromatography-ion mobility spectrometry (GC-IMS) coupled with chemometrics analysis.The results show that a total of 41 compounds were detected. The metabolic compositions between organic and non-organic milk exhibit a high degree of similarity, whereas the metabolic characteristics of Yak milk differed significantly from Holstein milk and Jersey milk. Some compounds were screened as differential compounds for distinguish organic milk from non-organic milk, including beta-ocimene, 2-butanone, 2[DK],5-dimethylfuran, propanol, 2-ethyl-6-methylpyrazine, and 5-methyl-3-heptanone. The study characterized the differential features of organic and non-organic milk, providing data to support the authenticity identification of organic milk.