In order to screen drug rapidly and effectively in vivo, the approach of the establishment for mouse melanoma model with primary cell from melanin solid tumor tissue of mouse is studied. The primary cell and the cultured B16 cells in vitro are implanted into two groups of C57BL/6 mice by hypodermic, respectively. The appearing time and the growth rate of tumor in two groups of mouse are investigated. The results show that when the inoculation is 0.2 mL for each one (the cell concentration of 5.0×10⁶/mL), the tumor formation rates of the primary tumor cells of mouse melanoma and in vitro cultured B16 cells are 100% and 80%, respectively, and it takes about 3 and 8 days respectively that the tumor sizes become about 4 mm³. The preliminary evaluation of two models are carried out with taxol, showing that the primary B16 cell model is a feasible and effective method for screening and evaluating the antitumor activity of specific compounds in vivo compared with the B16 cell model cultured in vitro, and it has shorter tumor forming time, higher tumor forming rate and less error in the obtained experimental data, which provides a new way for the development and research of related drug evaluation models.