The environmental pollution of perchlorate is more and more serious because of its widely usage. Several parameters affecting perchlorate degradation are examined through batch experiments by perchlorate-degrading bacteria, which include temperature, pH, the concentration of carbon source, ClO-₄, NO-₃, and water-soluble and water-insoluble quinone compounds. Perchlorate-degrading bacteria is collected and enriched from a sewage treatment plant. The results suggested that the optimal conditions for perchlorate biodegradation are 30 ℃, pH 7.5~8.5 and 2.4 g/L acetate. Different NO-₃ concentrations have significant effect on perchlorate biodegradation. The higher the initial concentration of ClO-₄, the faster the degradation rate. The acceleration effect of AQDS is the best among dissolved quinone compounds, and the optimal concentration is 1.44 mmol/L. The acceleration effects of 1,5-dichloroanthraquinone is best among non-dissolved quinone compounds, and the optimal concentration is 0.090 mmol/L.