Salidroside and p-tyrosol are the main active ingredients. Their partition behaviors are not clear in different solvent extraction systems. Separation of salidroside and p-tyrosol from Rhodiola Tibetica extract was investigated in different solvent extraction systems. Effect of extraction conditions such as pretreatment, pH and extractants on partition behaviors was also studied. Results showed that pretreatment of tannins removal from the extract can avoid emulsification during solvent extraction. For liquid-liquid solvent extraction, mass fraction of salidroside and p-tyrosol in tributyl phosphate (TBP) phase can reach 60% and 98%, respectively when pH is below 9.0. Purified salidroside solution can be obtained in the lower aqueous phase. For the three-liquid-phase system composed of TBP, PEG2000 and ammonium sulphate, salidroside is mainly partitioned into the middle phase and the top phase, 70% of salidroside in the middle phase. p-tyrosol is mainly partitioned into the top phase, above 65%, but its mass fraction in the bottom phase is below 5% .