An exposure experiment is carried out to study the effect of 0, 10%, 30%, 50% and 70% (V/V) streptomycin wastewater from a workshop on superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in muscles of zebra fish for 15 days. The results show that SOD activity is significantly induced on the third day, and the highest expression of SOD reaches 30.56 U/mgprot in 50% group. SOD activity in 70% group is gradually reduced and in a dose-time manner. MDA activity of 30% and 50% group are significantly induced on the third day and drop dramatically on the sixth day, namely 43.04% and 47.50%, respectively, while MDA activity is higher than the control group on the twelfth day and fifteenth day. During the exposure, MDA activity of 10% and 70% group are higher than the control group. It is suggested that SOD activity and MDA content in the exposure process chang apparently, and the streptomycin wastewater causes oxidative damage on zebra fish.