Ozone oxidation treatment for simulated acid red B dye wastewater was investigated. Orthogonal experiments and single factor experiments were applied to determine the optimal operating conditions on COD removal in reaction time, ozone flux, initial pH and reaction temperature. The results show that when the reaction time is 10 min, ozone flux is 10.53 mg/L, initial pH is 10.0, and acid red B mass is 250 mg/L, the acid red B and TOC removals can reach 99.31% and 44.91%, respectively. Ultraviolet-visible spectroscopy analysis shows that the conjugate system of acid red B molecules is effectively destroyed by ozone. The ozone oxidation degradation process of acid red B is approximately complied with the first-order kinetics equation ln(c₀/c_t) = 0.167 7t +1.624 7.