The effects of medias containing different concentration of IAA and 6-BA or NAA and 6-BA on tomato callus induction, bud differentiation and rooting were detected in hypocotyl and cotyledon of Zhenmu No.1. The results show that MS+0.2mg/L IAA+1.0mg/L 6-BA is the best medium for callus induction and bud differentiation of cotyledon；MS+0.5mg/L IAA+1.0mg/L 6-BA is the optimum medium for callus induction and bud differentiation of hypocotyl；the optimum root induction medium is MS +0.1mg/L IAA.