A new assay of micellar electrokinetic capillary chromatography with sweeping was developed to determine chlordimeform and semiamitraz chloride in honey. The factors affecting resolution and peak height were studied. The optimum condition for separation and enrichment is determined as that the running buffer consists of 30mmol/L borate, 20mmol/L sodium dodecyl sulfate and 20% (V/V) methanol whose pH is adjusted to 9.67. The sample is injected at 10kV for 90s, and the separation voltage is 20kV. Under the optimum condition, the peak enrichment factors of chlordimeform and semiamitrazchloride are 1100and 1000; the linear dynamic range is 0.03~0.25mg/L; the detection limits and the average recoveries of spiked sample are 0.88μg/kg and 91.8%~100.6% for chlordimeform, and 0.62μg/kg and 90.5%~102.6% for semiamitraz chloride, respectively. The assay can meet the requirements of determination of maximum residue limits regulated by a number of countries and organizations, and can be applied to determine the residues in honey.