A glucose oxidase electrode with SiO_2sol-gel immobilization was prepared. The optimum experimental conditions of enzyme electrode were investigated. The response characteristics of enzyme electrode and inhibition of heavy metal ions on the enzyme electrode were also discussed. The experimental results show the amprometric response to glucose of enzyme electrode is inhibited due to the existence of Hg²⁺ and Fe³⁺ when the working potential is 0.8V, pH is 5.0and the temperature is 25℃. There is a linear relationship between the inhibited amprometric response and certain concentration of heavy metal ions. The linear range of Hg²⁺ and Fe³⁺ are 0～1.5μmol/L and 0～60μmol/L.The recovery of response on the inhibited enzyme electrode is possible by immersing in EDTA solution after 10min.