This paper discusses the development of the firmware program and the application program in the data acquisition adapter based on USB2.0. Firstly, it simply introduces the design of the data acquisition adapter and especially the interface CY7C68013A chip supporting the USB；then according to the frame structure of its firmware program, it introduces the development of its firmware program in detail；finally, it introduces the design of driver program and the realization of the application program. It proves by practice that this adapter not only has high sampling accuracy, high velocity and good reliablility, but also has the characters of small volume, convenience and so on.