This paper investigates the conditions of immobilization of chitosan adopting sodium alginate, poly (vinyl alcohol) and glutin as carriers, and farther uses the immobilized chitosan to adsorb the Cu(Ⅱ)and Cr(Ⅵ)in water. The results show that when individually using 2% (w/w) sodium alginate, 1% poly (vinyl alcohol) (w/w) + 1%(w/w) sodium alginate, 1%(w/w) glutin + 1% (w/w) sodium alginate to immobilize chitosan, the glomeration and mechanical performance of the immobilized chitosan were the best. It also tests the effects of quality of chitosan, pH and the time of adsorption on the effectivity of adsorption. The results indicate that when 0.20g chitosan was mixed with 1% poly (vinyl alcohol) (w/w) + 1% sodium alginate (w/w), pH was 6.5and the time of adsorption was 8h, 99.89% Cu (Ⅱ) was removed; when 0.15g chitosan was mixed with 1% poly (vinyl alcohol) (w/w) + 1% sodium alginate (w/w), pH was 4.5and the time of adsorption was 10h, 98.64% Cr (Ⅵ) was removed.