The optimal ultrasonication-assisted extraction process of the xanthophyll from hibiscus manihot L flower is studied by the orthogonal test.It shows that the optimal process extracting xanthophyll from the flower is 800mL/L ethanol with petroleum aether-ethanol mixture, 45min for ultrasonic time,1∶20for ratio of material to liquid, granularity less than 100pore. The xanthophyll content is 4325μg/100g DW (dry-weight of flower) under these conditions.