水-有机混合介质中电流型生物传感器对葡萄糖的响应
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(1.河北科技大学理学院,河北石家庄 050018;2.河北医科大学基础医学院,河北石家庄 050017)

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任聚杰(1963-),男,河北石家庄人,教授,博士,主要从事电化学分析方面的研究

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河北省自然科学基金资助项目(B2010000844)


Response of amperometric biosensor to glucose in aqueous-organic mixed media
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(1.College of Sciences, Hebei University of Science and Technology, Shijiazhuang Hebei 050018, China;2. Basic Medical College, Hebei Medical University, Shijiazhuang Hebei 050017, China)

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    摘要:

    利用自组装单分子层(SAMs)/普鲁士蓝(PB)/壳聚糖(Chit)界面固定葡萄糖氧化酶(GOD),构建酶生物传感器,对传感器在极性非质子有机溶剂如乙腈(AN)、二甲基亚砜(DMSO)和N,N-二甲基甲酰胺 (DMF) 存在条件下的电化学特性进行了研究,考察了有机溶剂体积分数对传感器响应的影响。结果表明,传感器在50% (体积分数)AN中的电流响应可达到磷酸缓冲溶液(PBS)中的73%。传感器响应速度快、重现性好、抗干扰能力强,展现了高的灵敏度(57.3mA·M·cm-2)。该传感器用于血清标本中葡萄糖的检测,测定结果与市售葡萄糖酶光度测定试剂盒测定结果相吻合。

    Abstract:

    A biosensor was fabricated with glucose oxidase (GOD) immobilized on self-assembled monolayers (SAMs)/Prussian blue (PB)/chitosan (Chit). The electrochemical characteristics of the biosensor were studied in the presence of dipolar aprotic organic solvents such as acetonitrile (AN), dimethyl sulfoxide (DMSO) and N,N-dimethyl formamide (DMF). The effect of volume percentage of organic solvents on biosensor response was investigated. It is observed that the response current of the biosensor in 50% AN reaches 73% of that in phosphate buffer solution (PBS). The amperometric signals are fast, reproducible and exhibiting high sensitivity (57.3mA·M·cm-2). The biosensor was used to determine glucose in serum specimens, and the results are consistent with those obtained with the commercially available glucose enzyme photometric kit.

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引用本文

任聚杰,籍雪平,李献锐,王贝贝,王 娜.水-有机混合介质中电流型生物传感器对葡萄糖的响应[J].河北科技大学学报,2012,33(5):391-396

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  • 收稿日期:2012-06-18
  • 最后修改日期:2012-09-05
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  • 在线发布日期: 2013-08-29
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